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Time-resolved absorption difference profiles for WT PS I complex excited at 660 nm and probed at 390 nm. Negative-going signals indicate photobleaching/stimulated emission. (A) Time-resolved absorption difference profiles obtained for PS I complexes with open and closed RC. (B) Time-resolved (open-closed) absorption difference profile, optimized fit to it using Eq. 1, and signal contributions due to the formation of 
and 
