TABLE 3.

The kinetics parameter of 23-kDa protein at various temperatures

	kou(s−1)	(ml/mol)	kof (s−1)	(ml/mol)
Temperature (°C)*
3	3.9 × 10−6	−99.6	0.012	98.7
10	3.2 × 10−5	−71.9	0.860	101.6
20	1.3 × 10−4	−66.2	1.870	84.1
35	1.4 × 10−3	−56.6	1.990	63.4
45	2.7 × 10−2	−44.6	12.570	85.3
pH†
5.5	1.3 × 10−4	−66.2	1.870	84.1
7.0	3.06 × 10−5	−98.2	0.330	61.8
8.0	1.01 × 10−4	−104.2	2.560	84.7
9.0	3.2 × 10−3	−64.4	6.170	146.1

^*Protein at various temperatures was always at pH 5.5.

^†The temperature for experiments was at 20°C.