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Refolding of SBA and ConA as observed by gel filtration. The protein concentration used for the refolding study was 40 μM. The protein sample was denatured in 6 M GdnCl and then renatured by dialyzing in 25 mM HEPES buffer for 12 h. The samples were then loaded to a P-150 Biogel column (40 ml bed volume; void volume was 11 ml as determined by blue dextran). The flow rate was maintained at 5 ml/h throughout the experiment. The gel filtration was conducted at 293 K.
