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. 2004 Nov 8;88(2):1377–1386. doi: 10.1529/biophysj.104.047308

FIGURE 2.

FIGURE 2

SHG polarization from individual fibers. (a) To determine the intrinsic fibril ρ, regions are selected in which fibrils are all oriented parallel to the fiber axis (yellow mask). (b) The SHG polarization intensity is measured from these regions by imaging with an analyzer placed between the specimen and the forward SHG detector. The curves displayed are from a linearly polarized fundamental at 0° (red), 30° (black), 60° (green), and 90° (blue) to the fiber axis (colored arrows). Note the differences in images acquired with an analyzer oriented parallel (c) and perpendicular (d) to the fiber axis. In this case, the SHG is induced with a fundamental that is polarized perpendicular to the fiber axis. Scale bar = 50 μm.