FIGURE 3.

Lamellipodial membrane dynamics of CHO-EGFR cells. Representative short-timescale kymographs from time-lapse movies of CHO-EGFR cells stimulated with EGF and migrating on 1 μg/ml (A) and 30 μg/ml (B) Fn. Kymographs depict variations in lamellipodial activity along a one-pixel-wide line drawn perpendicularly to the cell membrane in sequential phase contrast images. Ascending contours show lamellipodial protrusion, whereas descending contours show lamellipodial retraction. Dark regions indicate membrane ruffling.