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. 2002 Sep 23;99(20):12651–12656. doi: 10.1073/pnas.202320599

Figure 2.

Figure 2

Spectra of green and red states of no. 9 (Kaede). (A) Absorption spectra of the unconverted green (green line) and converted red (red line) states. Spectra obtained during the green-red conversion are displayed as thin black lines. (B) G380 and G480: emission spectra of the green state (green line) on excitation at 380 and 480 nm, respectively. R340, R480, and R540: emission spectra of the red state (red line) on excitation at 340, 480, and 540 nm, respectively. The spectra of the green and red states were normalized with the highest values of G480 and R540, respectively. The small peak at 440 nm in G380 was caused by water raman and is indicated by *. (C) pH dependence of the green state absorbance. (D) pH dependence of the red state absorbance. (E) Band patterns of the green (G) and red (R) states of Kaede after SDS/PAGE and Coomassie brilliant blue staining.