Figure 6.

Rapid diffusion of red Kaede within the cytosol. (A) A fluorescence micrograph of a HeLa cell expressing green Kaede protein. The UV-exposed point is indicated by an open circle. (Scale bar: 20 μm.) (B) Representative images showing the emergence and spreading of the red fluorescence. Time after the end of the UV pulse is indicated in each picture. Images in A and B were taken by using the configuration shown in Fig. 4 A and B. (C) A series of fluorescence intensity-distance curves after the UV pulse. The fluorescence intensity along the line shown in the image at 0.03 s in B was analyzed. Each curve was fit by a Gaussian and is displayed. Time after the end of the UV pulse is indicated in the graph.