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. 2008 Oct 31;88(2):L14–L16. doi: 10.1529/biophysj.104.055442

Figure 1.

Figure 1

Steady-state anisotropy (r) was calculated from fluorescence emission spectra (425 nm excitation) for solutions containing the FRET donor, mCerulean (●) and a fusion protein containing the FRET acceptor, mVenus, linked to mCerulean (○). FRET between mCerulean and mVenus results in increased mCerulean (475 nm) anisotropy from a decreased fluorescence lifetime, and depolarized sensitized emission from mVenus (525 nm). Analysis of recombinantly generated proteins was performed as previously described (6).