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. 2004 Nov 19;88(3):1507–1523. doi: 10.1529/biophysj.104.043976

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Experimental and theoretical membrane P2Y₂ receptor fraction with respect to time. Experimental data show the time-course of P2Y₂-GFP in A7r5 cells (squares with mean ± SE for six cells) after application of 10 μM UTP at t = 0. The P2Y₂-GFP fluorescence has been taken to be proportional to the number of receptors in the membrane and has been scaled to become a percentage of the amount of receptor relative to that before agonist stimulation. The solid line is the theoretical time-course of the total surface receptor fraction, and the broken line is the time-course of the phosphorylated surface receptor fraction, both obtained by solving Eqs. 1 and 2.

Inline graphic — Experimental and theoretical membrane P2Y₂ receptor fraction with respect to time. Experimental data show the time-course of P2Y₂-GFP in A7r5 cells (squares with mean ± SE for six cells) after application of 10 μM UTP at t = 0. The P2Y₂-GFP fluorescence has been taken to be proportional to the number of receptors in the membrane and has been scaled to become a percentage of the amount of receptor relative to that before agonist stimulation. The solid line is the theoretical time-course of the total surface receptor fraction, and the broken line is the time-course of the phosphorylated surface receptor fraction, both obtained by solving Eqs. 1 and 2.