Table 2.
IC50 values for formation of pG8-UP1 complexes
| Competitor | IC50 | Competitor | IC50 | Competitor | IC50 |
|---|---|---|---|---|---|
| pG5TEL | 3 nM | pG5+C | >1 μM | pG3 | 1 μM |
| pG5+T | 4 nM | pG8 | 4 nM | poly(dG) | 100 nM |
| pG5 | 20 nM | pG4R | 40 nM | rG5 | 5 nM |
| pG5+A | 30 nM | pG4 | 50 nM | rG5+U | 1 nM |
| pG5+G | 40 nM | pG3R | 100 nM |
IC50 values, the concentrations of unlabeled competitor oligonucleotide at which the labeled pG8-UP1 complex yield is halved, were estimated from the inhibition curves when the relative amount of complex was plotted against the concentration of a competitor.