TABLE 1.

1 Effect of various model parameters on apparent off rate at 0.7 dyn/cm²

Parameter	Change	Off rate ± SE (s−1)	P-value
Single-bond base state		9.36 ± 1.68
Linear elasticity, k	0.25 → 0.5 pN/nm	14.34 ± 3.11	p = 0.162
Cytoskeletal unbinding,	1 → 0.5 s−1	15.46 ± 1.95	p < 0.05
Viscous tether stretch,	60 → 120 pN	33.74 ± 3.07	p < 0.001
Cell compression, P0	17 → 34 N/m2	16.04 ± 2.18	p < 0.05
Initial bond length, λ	80 → 160 nm	12.83 ± 1.45	p = 0.119
Double-bond base state (force shared equally)		4.32 ± 0.32
Double bonds, sequential force loading		12.05 ± 1.32	p < 0.001

Differences were tested using two-sample Z-test.