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A typical force-distance curve for polymeric DNA. Stretching curves were calculated using Eqs. 5 and 12 in Rouzina and Bloomfield (2001a). The flat region of the force-distance curve at 65 pN is commonly known as the B-S plateau. The DNA unbinds at forces of ∼150 pN (depending upon experimental details such as the pulling rate). The inset shows a schematic representation of a DNA molecule stretched between a surface and an AFM cantilever.
