TABLE 3.
Results of fluorescence lifetime measurements of the deprotonated chromophore forms with a TCSPC setup
| GFP mutant | t1 (ns) | A1 (%) | t2 (ns) | A2 (%) | 〈t〉 |
|---|---|---|---|---|---|
| wt-GFP | — | — | 3.20 (0.05) | 100 | 3.20 |
| S65G | 0.69 (0.15) | 44 (14) | 1.63 (0.21) | 56 (16) | 1.21 |
| E222Q | 0.64 (0.58) | 13 (4) | 2.48 (0.31) | 87 (17) | 2.24 |
| S65G/E222Q | 0.51 (0.15) | 39 (10) | 1.50 (0.14) | 61 (12) | 1.11 |
| T203V | — | — | 3.21 (0.05) | 100 | 3.21 |
| S65G/T203V | — | — | 2.65 (0.05) | 100 | 2.65 |
| T203V/E222Q | 0.64 (0.35) | 14 (4) | 3.42 (0.75) | 86 (16) | 3.03 |
| S65G/T203V/E222Q | 0.46 (0.17) | 51 (10) | 1.72 (0.30) | 49 (13) | 1.08 |
Excitation and detection wavelengths of λexc = 465–480 nm and λdet = 510–515 nm, respectively, were used. Values in parentheses represent the standard error.