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Insulin amyloid spherulites formed in the presence of various salts. Optical microscopy images of solutions of 1 mM insulin, incubated at pH 2 and 65°C for 24 h. (A) No added salt. (B–F) Solutions of 0.1 M ionic strength using (B) NaCl, (C) NaI, (D) Na3C6H5O7 (sodium citrate), (E) MgCl2, and (F) LiCl. Scale bar in all pictures, 100 μm. (G) Histogram of spherulite diameters, n = 575 in the absence of NaCl (black bars), n = 666 in the presence of 100 mM (shaded bars).
