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Binding affinities of L35 to COHbA in the presence/absence of other allosteric effectors as determined by changes in the intrinsic fluorescence of Hb
| Effectors | KD1 (mM) | KD2 (mM) | R2 |
|---|---|---|---|
| L35 | 0.41 | 0.94 | 0.998 |
| L35+IHP | 0.23 | 0.85 | 0.999 |
| L35+Cl− | 0.50 | 1.00 | 0.998 |
| L L35+Cl−+IHP | 0.47 | 1.02 | 0.998 |
Conditions: 1.0 g % COHbA, 50 mM HEPES, pH 6.35. The KD values and correlation coefficients (R2) are calculated from curve-fitting analysis using Sigma Plot.
