FIGURE 5.

Hop diffusion trajectories observed at various frame times. Typical 1000-frame trajectories simulated for a particle undergoing hop diffusion over 120-nm length square compartments with a probability of 0.008 to pass the compartment boundary at each attempt, which were observed at frame times of 0.025, 0.110, 0.2, 2, and 33 ms. Note that each trajectory contains 1000 determined positions, and thus the total length of each trajectory is different from each other. The scale is the same except for the 33-ms trajectory. With an increase of the frame time, like the case of confined diffusion, the determined points within a compartment become more centralized due to time-averaging during the frame exposure time (thus, more space between two compartments is formed), and the diffusion is dominated by the hops between the compartments. In the case of the 2-ms frame time, hop movement between the same two compartments is also apparent (directly seen in the trajectory). In addition, since the total length of the trajectory is 2 s, and the mean residency time is 23 ms, there may be ∼87 hops on average. Here, this number of hops occurs over 20 compartments.