FIGURE 8.

The most frequent forces for breaking P-selectin:PSGL-1 bonds measured in tests with the molecules immobilized on glass beads (solid triangles; data taken from Evans et al., 2004) are compared to the most frequent forces for membrane unbinding from the PMN cytoskeleton as described in article I (open circles; Evans et al., 2005). The parallel hierarchy of these failure events at fast loading rates accounts for the correlation between the onset of tether formation and subsequent termination by P-selectin dissociation. (Error bars denote means ± SE, which lie within the data symbols for the bead-bead tests.)