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. 2002 Sep 17;99(20):12727–12732. doi: 10.1073/pnas.202236699

Figure 2.

Figure 2

Promoter- and activator-specific requirements for BUR6. (A) Northern blot analysis of selected transcripts from wild-type (WT), bur6–1, and spt15–601 strains grown under uninduced and induced conditions as indicated. (B) Activation domain-specific requirement for BUR6 with LexA-activation domain fusions. BUR6+ and bur6–1 strains containing a LacZ reporter driven by a LexA-binding site and a plasmid expressing either the LexA DNA-binding domain or the LexA DNA-binding domain fused to the activation domains of Hap4, Gal4, and Gcn4. β-Galactosidase assays were performed in triplicate, with the average LacZ activity and standard deviations shown.