TABLE 1.
Summarized measurements for the LL-distances, LC-distances, and LCL-angles of homologous pericentric regions under different test conditions
| Quiescent | Stimulated | 15-Min repair | 30-Min repair | 60-Min repair | 120-Min repair | 300-Min repair | |
|---|---|---|---|---|---|---|---|
| Chromosome 1 | |||||||
| Number of nuclei analyzed | 97 | 223 | 158 | 197 | 349 | 310 | 180 |
| Associated centromeres | 17 (17.5%) | 29 (13.0%) | 42 (26.6%) | 94 (47.7%) | 150 (43.0%) | 151 (48.7%) | 48 (26.7%) |
| Nuclei in S-phase | 0 | 110 | 107 | 152 | 270 | 258 | 130 |
| Associated centromeres in S-phase | 0 | 22 (20.0%) | 31 (29.0%) | 82 (53.9%) | 133 (49.3%) | 137 (53.1%) | 43 (33.1%) |
| Mean LL-distance of homologous pericentric regions [dn]+STD | 0.39 ± 0.22 | 0.4 ± 0.2 | 0.28 ± 0.22 | 0.26 ± 0.21 | 0.28 ± 0.22 | 0.26 ± 0.22 | 0.30 ± 0.19 |
| Mean LC-distance of pericentric regions to nuclear center [dn]+STD | 0.32 ± 0.10 | 0.29 ± 0.10 | 0.28 ± 0.09 | 0.26 ± 0.11 | 0.27 ± 0.12 | 0.25 ± 0.09 | 0.26 ± 0.11 |
| Mean LCL-angle of pericentric regions [°] | 77.82 | 73.76 | 54.36 | 49.88 | 65.51 | 60.05 | 66.44 |
| Chromosome 15 | |||||||
| Number of nuclei analyzed | 99 | 387 | 107 | 214 | 295 | 345 | 197 |
| Associated centromeres | 12 (12.1%) | 133 (34.4%) | 58 (54.2%) | 117 (54.6%) | 139 (47.1%) | 150 (43.5%) | 87 (44.2%) |
| Nuclei in S-phase | 0 | 155 | 87 | 187 | 247 | 273 | 165 |
| Associated centromeres in S-phase | 0 | 108 (69.7%) | 50 (57.4%) | 107 (57.2%) | 127 (51.4%) | 132 (48.4%) | 77 (46.7%) |
| Mean LL-distance of homologous pericentric regions [dn]+STD | 0.35 ± 0.17 | 0.3 ± 0.21 | 0.21 ± 0.18 | 0.22 ± 0.2 | 0.24 ± 0.19 | 0.26 ± 0.2 | 0.25 ± 0.19 |
| Mean LC-distance of pericentric regions to nuclear center [dn]+STD | 0.27 ± 0.10 | 0.27 ± 0.10 | 0.27 ± 0.09 | 0.25 ± 0.09 | 0.23 ± 0.09 | 0.24 ± 0.10 | 0.24 ± 0.11 |
| Mean LCL-angle of pericentric regions [°] | 88.03 | 64.71 | 40.21 | 55.59 | 56.90 | 51.12 | 50.66 |
| Chromosome X | |||||||
| Number of nuclei analyzed | 212 | 239 | 133 | 266 | 314 | 470 | 135 |
| Associated centromeres | 26 (12.3%) | 71 (29.7%) | 53 (39.9%) | 110 (41.4%) | 125 (39.8%) | 197 (41.9%) | 58 (42.9%) |
| Nuclei in S-phase | 0 | 143 | 124 | 216 | 253 | 331 | 118 |
| Associated centromeres in S-phase | 0 | 54 (37.8%) | 52 (41.9%) | 101 (46.8%) | 109 (43.1%) | 156 (47.1%) | 50 (42.4%) |
| Mean LL-distance of homologous pericentric regions [dn]+STD | 0.4 ± 0.2 | 0.35 ± 0.24 | 0.28 ± 0.21 | 0.28 ± 0.22 | 0.29 ± 0.23 | 0.21 ± 0.19 | 0.29 ± 0.21 |
| Mean LC-distance of pericentric regions to nuclear center [dn]+STD | 0.31 ± 0.09 | 0.32 ± 0.10 | 0.34 ± 0.10 | 0.27 ± 0.10 | 0.29 ± 0.08 | 0.29 ± 0.09 | 0.31 ± 0.10 |
| Mean LCL-angle of pericentric regions [°] | 90.41 | 68.57 | 55.88 | 59.98 | 65.97 | 67.44 | 58.15 |