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. 2005 Jan 14;88(3):2340–2349. doi: 10.1529/biophysj.104.041483

FIGURE 1.

FIGURE 1

Time-resolved TMRE fluorescence of single mitochondria in response to addition of malate. TMRE fluorescence was monitored in the absence of ADP. (A and B) ΔΨm-dependent fluorescence images of TMRE in single mitochondria. The time interval between images is 3 s. (A) The arrow marks the addition of malate (5 mM). (B) The arrow marks the rapid depolarization observed after addition of malate. (C) The typical responses of ΔΨm observed in single mitochondria to addition of malate. The vertical axis represents the fluorescence intensity of TMRE in single mitochondria, normalized by the TMRE fluorescence in buffer. At t = 0, 5 mM malate was added (arrow). The fluctuations of ΔΨm significantly depend on individual mitochondria. Mitochondria 1–3 are fluctuating, whereas mitochondrion 4 does not show the fluctuation of ΔΨm.