Table 1.
Human β-globin mRNA decay products
| Distance, nt
|
Cleavage site | ||
|---|---|---|---|
| Primer extension | S1 nuclease mapping | Electrophoretic mobility | |
| 67 | 67 | 67 | cuga |
| 77 | 77 | gucu | |
| 93 | 92 | cugu | |
| 143 | 143 | gcug | |
| 167 | 167 | ccag | |
| 187 | 186 | 186/187 | uugg |
| 205 | cuga | ||
| 214 | 214 | guua | |
| 259 | 259 | gugc | |
| 269 | 269 | guga | |
| 272 | augg | ||
| 276 | 276 | cugg | |
| 282 | ucac | ||
| 316 | 316 | ugag | |
| 324 | 324 | 324 | cugc |
Numbers refer to distances of the cleavage sites from the 5′ end of full-length mRNA as determined by using primer extension or S1 nuclease mapping of Thal 10 MEL-cell RNA, or electrophoretic mobility of in vitro-labeled human β-globin RNA incubated with polysome extract from uninduced or DMSO-induced MEL cells. Endonucleolytic cleavage sites are more accurately derived (±2 nt) by using primer extension and electrophoretic mobility assays.