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. 2005 Jan 28;88(4):2452–2461. doi: 10.1529/biophysj.104.055541

FIGURE 2.

FIGURE 2

X-ray structure of the anionic (B-state) GFP reveals a hydrogen-bond escape pathway for the proton, leading from the chromophore (Tyr-66) to the OH of Thr-203 and then to the bb-carbonyl of His-148, which is already on the surface of the protein. The HB lengths (O⋯O distance) are given in angstroms (for comparison, the typical distance for liquid water is 2.85 Å). Color codes: oxygen, red; nitrogen, blue; carbon, gray; and added hydrogens, white; bb connections, black. Coordinates from PDB file 1Q4A; x-ray diffraction measurements of Jain and Ranganathan (2004).