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GPex values of Laurdan in PCPE and PSM bilayers as a function of temperature. Laurdan emission spectra in PCPE vesicles were recorded stepwise by 5°C between 35°C and 85°C, in PSM vesicles between 10°C and 65°C. Vesicles contained 1 mol % Laurdan, and the GPex value was calculated as described above.
