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. 2005 Jan 14;88(4):2670–2680. doi: 10.1529/biophysj.104.057059

FIGURE 2.

FIGURE 2

13C-NMR assessment of the transbilayer distribution of SPM and POPC from the headgroup choline methyl resonances. On the basis of sensitivity to Mn2+ (Panel A), the upfield choline methyl resonance (54.9 ppm) was assigned to outer leaflet SPM/POPC, whereas the resonance at 54.5 ppm was assigned to inner leaflet choline methyl residues of SPM/POPC; the latter signal was not affected by external Mn2+. Panel B shows the choline resonances of the outer and inner leaflets of POPC/SPM/PE (45:45:10) SUVs in the absence of Mn2+.