FIGURE 3.
Videomicrographs of GUVs tested by micromanipulation after exposure to Aβ 1–42 peptide. Giant unilamellar vesicle composed of POPC (top) and composed of POPC/SM/Chol (1:1:1 molar ratio, bottom) held under constant, low membrane tension exposed to 5 μM Aβ 1–42 peptide. (A) Before and (B) after peptide exposure. τlyse is the critical tension to lyse the vesicle (mN/m) and is shown in the figure to denote the change in the membrane physical properties under the different conditions. It is the average tension when the vesicle breaks as the suction pressure was increased. Videomicrographs were taken optimizing the brightness and contrast setup to set the best condition for vesicle vision. The size bar at bottom-right of each panel indicates the scale of the pictures (5 μm). Inserted bars indicate the diameter of GUVs and the projection toward the inner of the suction pipette after peptide interaction.