Figure 2.

High-throughput drug screening and kinetic analysis

(A) Analysis of the thermal stability of the Hcp protein in combination with other drugs. The drug corresponding to each curve is detailed in Table 1.

(B) Analysis of the interaction between the Hcp protein and drugs via BIL. Bio-layer interferometry (BLI) is a label-free technology for analyzing biomolecular interactions, based on the principle of optical interference. BLI enables real-time monitoring of binding events and provides key data such as binding affinity. The Y axis represents the response value (nm), which quantifies the spectral shift caused by changes in biomolecular layer thickness. The response-over-time curve reflects the real-time processes of molecular “association” and “dissociation.”

(C) Drugs can inhibit the growth of B. pseudomallei (HNBP001) in vitro.

(D) Kinetics analysis of the Hcp protein and netilmicin sulfate, using BLI.