FIGURE 5.

Distribution of the UV laser induced hot piperidine sensitive lesions: double-stranded versus single-stranded DNA. Oligonucleotides-II, either double-stranded (lines 2, 4, 6, 8, 10, 12 (A) and top frame (B)) or single-stranded (lines 1, 3, 5, 7, 9, 11 (A) and bottom frame (B)) were submitted to UV laser radiation at different pulse intensities and analyzed by sequencing gel electrophoresis after hot piperidine treatment. (A) Gel; (B) quantified quantum yields Q_pip as a function of the laser pulse dose and fitted by Eq. 2a. Irradiation conditions were similar to those in Fig. 4.