FIGURE 5.

Analysis of a bilayer compositional array by fluorescence and secondary ion signals collected by the NanoSIMS 50. A gradient between egg PC (containing 0.5 mol% TR-DHPE) and D₃₁-POPC was formed by depositing one droplet of each of two different vesicle suspensions (egg PC and D₃₁-POPC) onto a FN-patterned surface. (A) Fluorescence image showing the variation in composition, from low fluorescence to high fluorescence. (B) ¹²CD⁻/¹²C⁻ SIMS signal from the same region shows the ¹²CD⁻/¹²C⁻ signal is maximal where the fluorescence is minimal (the total dwell time was 8 ms). (C) Normalized ¹²CD⁻/¹²C⁻ and ¹²C¹H⁻/¹²C⁻ signals versus the fluorescence, where each of the eight data points on each line corresponds to one of the eight corrals within the lower row highlighted with arrows in panels A and B. The best fit to a linear relationship is plotted through the points.