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. 2005 Jan 21;88(4):3050–3059. doi: 10.1529/biophysj.104.053546

FIGURE 2.

FIGURE 2

Effect of different concentrations of ficoll applied to the cytoplasmic side of MscS on the channel currents. (A) Ficoll changed reversibly the activities of the MscS channels, fewer channels opened, and the inactivation was faster. All traces were recorded from the same patch. Arrows indicate application and release of suction (downward and upward, respectively), and c marks the closed level of the channels. All traces were recorded at +15 mV and suction pulse was 215 mm Hg. The effect of 2% and 5% of ficoll on the inactivation time constant, τ, (B), and on total current, I, are shown as a ratio I/Imax, where Imax represents the current through all the channels in the patch (C). (D) The effect of 2% and 5% of ficoll on the channel open probabilities, Po, plotted against suction and fitted with Boltzmann curves. Data sets presented in B, C, and D represent one experiment.