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Effect of PEG 200 on the MscS currents was time-dependent. After introducing PEG 200 into the experimental chamber, we observed the initial peaklike response (B), which disappeared in the course of the experiment (C). For explanation, see the text. All traces were recorded from the same patch at +15 mV. Suction pulse (indicated by the downward arrow) was 155 mm Hg and its duration was 12 s. c marks the closed level of the channels.
