Figure 1.

BNIP3 induction correlates with increased apoptosis. (A) Cardiac myocytes were subjected to hypoxia with (Left) or without (Right) medium change, harvested at the indicated times, and processed for genomic fragmentation assays. The pH of the media at the time of harvesting is shown at the bottom. (B) Northern blots of cardiac myocyte RNA extracted from hypoxic cultures. (C) Western blot analysis of proteins from hypoxic cardiac myocytes as in A. Anti-BNIP3 recognizes two bands at approximately 60 and 30 kDa, corresponding to SDS-resistant homodimers and monomers, respectively. Lower gels show the same blot probed with anti-Bax, Bak, and β-actin. Results are representative of at least three experiments.