Figure 2.

BNIP3 antisense inhibits programmed death of cardiac myocytes. (A) Cultures were incubated with BNIP3 (AS) or random sequence (R) oligonucleotides (ON) as described in Materials and Methods, subjected to hypoxia-acidosis as indicated, and analyzed for DNA fragmentation. (B) Cardiac myocytes were treated with oligonucleotides as in A, and extracted proteins were analyzed by Western blots with anti-BNIP3 and β-actin. Results are representative of three experiments.