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Acid-mediated opening of MPTP in BNIP3-transfected myocytes. Cardiac myocytes were cotransfected with pGFP and BNIP3 or BNIP3 Δ-TM, as indicated. After 48 h, transfected cultures were exposed to continued culture at neutral pH or to acidosis for an additional 8 h as described in Materials and Methods. Cultures were stained with MitoTracker Red dye, fixed, and analyzed by microscopy. At least 50 GFP-positive myocytes per condition were scored from four separate dishes.
