Table 2.
Distribution of Man II in Golgi cisternae and vesicles in different experimental conditions
| Experimental conditions (fixation/dilution of antibody and length of incubation) | No. of stacks | % of total labeling in peri-Golgi vesicles | Labeling density, gold per μm
|
||
|---|---|---|---|---|---|
| Cisternae | Vesicles | Ratio cisternae/vesicles | |||
| PAF 2% + Glut. 0.2%/1:20 1 h | 40 | 2.0 | 2.31 | 0.13 | 17.8 |
| PAF 2% + Glut. 0.2%/1:50 1 h* | 184 | 5.3 | 0.56 | 0.15 | 3.7 |
| PAF 2% + Glut. 0.2%/1:100 2 h | 48 | 6.4 | 0.91 | 0.25 | 3.6 |
| PAF 1% + Glut. 0.5%/1:50 1 h | 65 | 1.4 | 1.22 | 0.13 | 9.4 |
| PAF 1% + Glut. 0.5%/1:100 1 h | 21 | 1.8 | 0.90 | 0.06 | 15 |
| PAF 1% + Glut. 0.5%/1:100 ON | 40 | 2.5 | 2.07 | 0.17 | 12.2 |
PAF, paraformaldehyde; Glut., glutaraldehyde; ON, overnight.
*
For comparison the experiments described in Table 1 are summarized and indicated in bold characters.