TABLE 1.
SLS results for lysozyme
| pH | z | I | 2B2 | z2/2ρ1 | ß22 |
|---|---|---|---|---|---|
| 5.0 | 10.2 | 0.015 | 117.7 | 169.6 | −51.9 |
| 5.0 | – | 0.030 | 63.0 | 84.8 | −21.8 |
| 5.0 | – | 0.060 | 36.7 | 42.4 | −5.7 |
| 7.0 | 8.0 | 0.007 | 165.0 | 223.6 | −58.6 |
| 7.0 | – | 0.022 | 57.0 | 71.1 | −14.1 |
| 7.0 | – | 0.052 | 25.1 | 30.1 | −5.0 |
| 9.0 | 6.7 | 0.007 | 100.5 | 156.8 | −56.3 |
| 9.0 | – | 0.022 | 31.0 | 49.9 | −18.9 |
| 9.0 | – | 0.052 | 13.6 | 21.1 | −7.5 |
B2, z2/2ρ1, and ß22 are in units of ×104 mol ml/g2. I is the ionic strength (including buffer) in mol/liter; z is the charge carried by the protein, obtained from titration experiments (Kuehner et al., 1999). An average Mw = 14,300 Da is used in the calculations.