Skip to main content
. 2002 Sep 20;99(20):12853–12858. doi: 10.1073/pnas.202115499

Figure 1.

Figure 1

Oligomerization of Pma1 is dependent on Lcb1. Cells were induced to synthesize tagged Pma1 by shifting to galactose medium for 4 h at 30°C, as described in Methods and Materials. Lysate was prepared, native IPs were performed with anti-HA and anti-myc antibodies and normalized to lysate protein, and were analyzed by Western blotting with the same antibodies. (A) Lysates were prepared from wild-type (F1105) and lcb1-100 (RH3804) cells expressing HA-Pma1 (pND542) and myc-wild-type Pma1 (pWQ3). (B) Lysates were prepared from F1105 cells bearing only GAL-HA-PMA1 (pND542) (1) or both GAL-HA-PMA1 and GAL-myc-pma1-D378N (pWQ4) (2). (C) Lysates were prepared from LCB1+ (F1105) and lcb1-100 (RH3804) cells expressing both HA-Pma1 (pND542) and myc-Pma1-D378N (pWQ4). Arrowheads indicate Pma1; IgG bands migrate below Pma1.