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Ubiquitylation of Pma1. LCB1+ (F1105) and lcb1-100 (RH3804) cells bearing GAL1-HA-PMA1 (pND542) alone or with GAL1-myc-pma1-D378N (pWQ4) were shifted to galactose-containing medium for 4 h. IPs under denaturing conditions with anti-HA or anti-myc were assayed by Western blotting with anti-ubiquitin. Arrow shows position of Pma1.
