FIGURE 7.
Large oil droplets were observed in the aqueous phase in the cuvette containing buffer/pyrene/SC3 (100 μg/ml)/paraffin oil (A and B). After the membrane was formed overnight at the interface, the solution was slowly stirred for 2.5 h and an aliquot of the aqueous solution was taken for microscopic measurement. No droplets were observed in a control sample using 0.01% (v/v) Triton X-100 instead of SC3 (C and D). Samples were visualized by light microscopy (A and C) and fluorescence microscopy (B and D). Scale bar, 10 μm.