FIGURE 2.

(A) Comparison of the raw anisotropy decays of fl-aptamer and TR6-aptamer at 25°C. The anisotropy decays were calculated from parallel and perpendicular polarized fluorescence decays. (B) Plot of the raw anisotropy data for the TAMRA-aptamer at 25°C (solid line) along with theoretical plots of the anisotropy decay for 0, 50, and 100% amplitudes of the mobile state for the long-lifetime distribution according to Eqs. 4–6 (dashed lines). For the long-lifetime distribution, the immobile state (coupled to the DNA) has a rotational correlation time of 5 ns and the mobile state has a rotational correlation time of 0.5 ns. The short-lifetime distribution is assumed to be coupled to the DNA with a rotational correlation time of 5 ns. Data at early times are omitted due to irregularities caused by the instrument response function (see Materials and Methods).