TABLE 1.

Fluorescence anisotropy parameters at 25°C

Sample	φ1 (ns)	β1	φ2 (ns)	β2	χ2
20 nM TR-cadaverine*†	0.23 ± 0.02	1.0	–	–	1.38
10 nM TR12-aptamer*‡	0.7 ± 0.2	0.27 ± 0.05	5.1 ± 0.4	0.73 ± 0.05	1.68
20 nM TR12-aptamer*‡	0.5 ± 0.2	0.28 ± 0.04	5.0 ± 0.4	0.72 ± 0.04	1.91
50 nM TR12-aptamer*‡	0.6 ± 0.2	0.27 ± 0.05	4.9 ± 0.4	0.73 ± 0.05	1.13
20 nM TR6-aptamer*‡	0.5 ± 0.2	0.26 ± 0.04	5.1 ± 0.4	0.74 ± 0.04	1.56
200 nM fl-aptamer§†	0.49 ± 0.03	1.0	–	–	2.70
50 nM TAMRA-aptamer (lifetime-associated anisotropy)¶‖					1.41
0.14-ns component		1.0	–	–
0.63-ns component		1.0	–	–
2.6-ns component

^*Samples excited at 590 nm and emission collected at 590 nm.

^†Anisotropy fitting function is: r(t) = r₀ exp(−t/φ).

^‡Anisotropy fitting function is: r(t) = r₀ [β₁ exp(−t/φ₁) + β₂ exp(−t / φ₂)].

^§Samples excited at 440 nm and emission collected at 612 nm.

^¶Samples excited at 563 nm and emission collected at 590 nm.

^‖Lifetime-associated anisotropy fitting was accomplished as described in Materials and Methods.