TABLE 2.

Fluorescence anisotropy parameters for 10 nM TR12-aptamer-IgE binding (25°C)

[IgE]*†‡	φ1 (ns)	β1	φ2 (ns)	β2	φ3 (ns)	β3	χ2
0 nM	0.5 ± 0.2	0.28 ± 0.04	5.0 ± 0.4	0.72 ± 0.04	–	–	1.91
5 nM	0.5 ± 0.2	0.26 ± 0.06		0.61 ± 0.05	50§	0.14 ± 0.07	1.47
10 nM	0.7 ± 0.3	0.26 ± 0.11		0.50 ± 0.08	50§	0.24 ± 0.13	1.91
15 nM	1.0 ± 0.5	0.25 ± 0.16		0.39 ± 0.13	50§	0.36 ± 0.19	1.43
26 nM	0.5 ± 0.3	0.17 ± 0.12		0.28 ± 0.07	50§	0.54 ± 0.14	1.74
150 nM	0.7 ± 0.3	0.23 ± 0.07		0.28 ± 0.05	50§	0.49 ± 0.09	1.55

^*All solutions are in binding buffer.

^†Samples are excited at 590 nm and emission is collected at 612 nm.

^‡Anisotropy fitting function is: r(t) = r₀ Σ β_i exp(−t/φ_i).

^§This rotational correlation time is fixed to account for overall protein rotation.