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Superposition of 3D structures of initial and final structures of (a) Kt-58 and (b) Kt-U4. The x-ray structures are shown in blue (more bent for Kt-58 and more open for Kt-U4) and representative final structures (averaged from MD; step, 500 ps) in red. The C-stem is overlaid. The changes from initial to final geometry were permanent in the simulated timescale (PDB files are available in Supplementary Material).
