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. 2002 Sep 13;99(20):12859–12864. doi: 10.1073/pnas.192264999

Figure 2.

Figure 2

IFN-γ-stimulated expression of the IRF1 gene does not require p38. p38α(+/+) and -(−/−) cells were treated with IFN-γ for 1 or 2 h (A) or treated with SB203508 for 30 min before stimulation with IFN-γ for 60 min (B), and total RNA was isolated. After RT, the amounts of IRF1 cDNA were assayed by using real-time PCR in duplicate in three independent experiments. The values of IRF1 expression after treatment with IFN-γ alone (IFNg) or with both SB203580 and IFN-γ (SB/IFNg) were normalized to those of untreated cells.