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. 2002 Sep 13;99(20):12859–12864. doi: 10.1073/pnas.192264999

Figure 3.

Figure 3

Anisomycin treatment enhances IFN-γ-induced transcription in a p38-dependent way. (A) p38α(+/+) and -(−/−) cells were treated with IFN-γ for 60 min with (an/IFNg) or without (IFNg) 20-min pretreatment with anisomycin or for 80 min with anisomycin alone (an), or left untreated (−). Total RNA was isolated, and the expression of IRF1 was determined in three independent experiments by using real-time RT-PCR carried out in duplicate (a representative result is shown). (B) p38α(+/+) cells were treated with anisomycin for 60 min (An) or with IFN-γ for 30 min (IFNγ) or pretreated with anisomycin (100 ng/ml) for 30 min followed by treatment with IFN-γ (30 min) (An + IFNγ). Tyr-701 phosphorylation (Upper) of STAT1 and equal loading (Lower) were assayed in whole-cell extracts by Western blotting by using pTyr701-S1 and STAT1 N-terminal antibodies, respectively.