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. 2002 Sep 13;99(20):12859–12864. doi: 10.1073/pnas.192264999

Figure 5.

Figure 5

NF-κB is not required for p38-mediated transcriptional enhancement. (A) p38α(+/+) and -(−/−) cells were treated for 60 min with TNFα, IFN-γ, or both, and the expression of IRF1 was determined. (B) Glioblastoma cells stably transfected with dominant negative IκB (GL-IκB-DN) or vector control (GL-Neo) were treated with IFN-γ with or without 20-min pretreatment with anisomycin. RNA was isolated, and the expression of IRF1 was analyzed. In both A and B, the expression was determined in two independent experiments by using real-time RT-PCR carried out in duplicate (representative results are shown). (C) GL-IκB-DN and GL-Neo cells were treated with IFN-γ with or without 20-min pretreatment with anisomycin, TNFα (25 min), or left untreated. Whole-cell extracts were prepared and analyzed by Western blotting for degradation of IκB by using IκB antibodies. The endogenous IκB and mutant IκB-DN bands are indicated.