Stern-Volmer plots of acrylamide quenching of the fluorescence of labeled protein variants with and without particles. (a) AEDANS-H10C, (b) AEDANS-T37C, and (c) AEDANS-S152C. The protein free in solution (□) and after 5 min incubation with silica nanoparticles at pH 6.3 (▪), 7.3 (Δ), 8.3 (○), and 9.3 (•).