TABLE 2.

Results of fluorescence anisotropy and lifetime measurements in position 10

	Free protein			Adsorbed to 15 nmp
pH	rs	τs (ns)*	φc (ns)	rs	τs (ns)*	φc (ns)
6.3	0.14	11.7	6.4	0.25	16.9	27.5
7.3	0.12	12.8	5.2	0.24	16.8	24.4
8.3	0.13	11.4	5.4	0.21	15.2	17.1
9.3	0.11	11.4	4.6	0.14	12.2	6.6

^*The lifetime is the average of a double-exponential decay (see Materials and Methods).