FIGURE 3.

STICS analysis results for computer simulations of two populations, one flowing (v_x = −0.12 μm/s and v_y = 0.08 μm/s) and one diffusing (varying diffusion coefficients). (A) The x velocity as resolved by STICS with and without the immobile population filtering. (B) The y velocity as resolved by STICS with and without the immobile population filtering. On both graphs, each point and error bar represents the average result of 100 simulations with standard deviation. The shaded regions show the set velocities in the simulation with an acceptable error of ±10%. The computer simulations were 128 × 128 pixels with 100 frames at a density of 100 particles/μm², using 0.1 s/frame, 0.06 μm/pixel, and an e⁻² radius of 0.4 μm.