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. 2005 Feb 18;88(5):3601–3614. doi: 10.1529/biophysj.104.054874

FIGURE 9.

FIGURE 9

Two-photon STICS velocity map image of EGFP/α-actinin flux in the vicinity of a retracting lamellar extension in a CHO cell plated on fibronectin. The original image-series reveals that the extended lamellar protrusions are retracting. Selecting regions of 16 × 16 or 32 × 32 pixels and performing the STICS analysis reveals a net flow of the α-actinin toward the cell interior (arrows). The spatial scale is shown as a bar and the velocity scale is shown as an arrow. The original image-dimensions are 512 × 480 pixels at 0.118 μm/pixel and a total of 120 images at 5 s/frame.