Figure 4.

Quantitative RT-PCR on the different genes of the albumin family. Embryos (15.5 E) from intercrosses matings were dissected and genotyped, and total RNA from their livers was extracted. The concentration of Gapdh transcript was measured for each sample tested, and the ratio of the tested transcript [Afp, albumin (Alb), alpha-albumin (Alf)] on the Gapdh transcript was calculated. The relative amounts the three mRNAs tested is given for wild-type embryos (Afp+/+), heterozygous (Afp+/−), or homozygous (Afp−/−). The number of each sample tested for each genotype is indicated in parentheses.